Hussen Abdu
University of Rwanda
Abstract Title:
Ameliorative Potential of Moringa Stenopetala (Baker f.) Cufod. (Moringaceae) Leaf Extract on Acrylamide-Induced Hematological and Hepatorenal Toxicity in Rats: An In vivo Toxicological Study
Biography:
Dr. Hussen Abdu Muhidin earned his Master's and PhD degrees in Anatomy from Addis Ababa University, College of Health Sciences. He is currently a Senior Lecturer at the University of Rwanda, where he is actively engaged in teaching, research, and academic mentorship. Previously, he served at Wollo University in several academic and leadership positions, contributing significantly to institutional development. Dr. Hussen has authored more than 25 peer-reviewed scientific publications in reputable international journals. He also serves as a reviewer and editorial board member for several respected journals, including PLOS ONE and BMC journals, reflecting his commitment to advancing scientific research and academic excellence.
Research Interests:
Moringa stenopetala (M. stenopetala) is an herbal plant used for nutritional, pharmacological, and industrial applications that could mitigate toxicity. People working in factories are exposed to acrylamide, a widely used industrial chemical; however, it has nephrotoxicity, hepatotoxicity, neurotoxicity, genotoxicity, and carcinogenic effects. This study examined the ameliorative effects of M. stenopetala leaf extract on acrylamide-induced hepatorenal toxicity in rats. Moringa stenopetala leaves were dried, ground into powder, mixed with 70% ethanol, then filtered using Whatman filter paper, and evaporated using rotavapor. Acrylamide crystals were purchased. Forty rats were divided into four groups, each containing 10 rats. Group I was given distilled water. Group II was treated with 15 mg/kg/day of acrylamide, Group III was treated with acrylamide plus M. stenopetala (500 mg/kg/day), and Group IV was treated with M. stenopetala leaf extract only. After 14 days of experiment, the animals were sacrificed, the blood sample was taken, and the liver and kidneys were prepared for histopathological examination. All results were evaluated by a one-way ANOVA using SPSS version 26. In animals exposed to acrylamide, a significant reduction in hemoglobin (9.8±0.6g/dL) and RBC levels (4.4±0.3 million/µL), and increased WBC levels (11.3±0.7 thousand/µL) were observed. In addition, microscopic evaluations revealed severe liver and kidney injuries. However, in animals treated with acrylamide and M. stenopetala leaf extract, hemoglobin and RBC levels increased, WBC count decreased, and liver and kidney tissue integrity showed improvement. Moringa stenopetala leaf extract has protective effects on alterations in blood parameters and tissues of the liver and kidneys caused by chemical exposure. This suggests the potential of the plant as a natural therapeutic agent for chemical-induced toxicity and tissue insults, and workers in a factory could use Moringa to mitigate such toxicity.
Keywords: Acrylamide; Hematological; Kidney; Liver; Moringa stenopetala; Toxicity
